Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. Conidiogenesis had been intensively studied in Aspergillus nidulans and regulatory pathway genes have been known to regulate conidiogenesis in stage specific manner. We reported the functional analyses of flbD, abaA, and wetA orthologs in F. graminearum. To understand genome-wide transcriptional profiling of conidiation, we employed RNA-seq of the wild-type Fusarium graminearum Z-3639 and each gene deletion mutants with three time courses (0 h, 6 h and 12 h after induction of conidiogenesis). AbaA experiment: 6 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum Z-3639 wild type and ΔabaA(ΔabaA::gen) mutant strains WetA experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔwetA(ΔwetA::gen) mutant strains flbD experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔflbD(ΔflbD::gen) mutant strains
Project description:Mitogen-activated protein kinases (MAPKs) are fundamental components of the plant immune system. MAPK cascades are activated by microbial elicitors and effectors, such as microbe-associated molecular patterns (MAMPs) and damage-associated molecular patterns (DAMPs). The activation of MAPKs plays essential roles in early and late responses associated with plant defense and innate immunity. In the present experiment, we investigated the role of HvMPK3 in the resistance of barley (Hordeum vulgare) against Fusarium graminearum. The proteomes of TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) were compared with wild types 24 h after the treatment with F. graminearum. Results explain the resistance of HvMPK KO lines to the pathogen.
