Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq Analysis of FlbD-, AbaA-, and WetA-dependent Genes in Fusarium graminearum Z-3639


ABSTRACT: Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. Conidiogenesis had been intensively studied in Aspergillus nidulans and regulatory pathway genes have been known to regulate conidiogenesis in stage specific manner. We reported the functional analyses of flbD, abaA, and wetA orthologs in F. graminearum. To understand genome-wide transcriptional profiling of conidiation, we employed RNA-seq of the wild-type Fusarium graminearum Z-3639 and each gene deletion mutants with three time courses (0 h, 6 h and 12 h after induction of conidiogenesis). AbaA experiment: 6 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum Z-3639 wild type and ΔabaA(ΔabaA::gen) mutant strains WetA experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔwetA(ΔwetA::gen) mutant strains flbD experiment: 3 samples examined: 0 h, 6 h and 12 h after induction of conidiogenesis of Fusarium graminearum ΔflbD(ΔflbD::gen) mutant strains

ORGANISM(S): Fusarium graminearum

SUBMITTER: Hokyoung Son 

PROVIDER: E-GEOD-46133 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

AbaA regulates conidiogenesis in the ascomycete fungus Fusarium graminearum.

Son Hokyoung H   Kim Myung-Gu MG   Min Kyunghun K   Seo Young-Su YS   Lim Jae Yun JY   Choi Gyung Ja GJ   Kim Jin-Cheol JC   Chae Suhn-Kee SK   Lee Yin-Won YW  

PloS one 20130910 9


Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops such as wheat, barley, and maize. Both sexual (ascospores) and asexual (conidia) spores are produced in F. graminearum. Since conidia are responsible for secondary infection in disease development, our objective of the present study was to reveal the molecular mechanisms underlying conidiogenesis in F. graminearum based on the framework previously described in Aspergillus nidulans. In this s  ...[more]

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