Dataset Information


Arabidopsis thaliana

ABSTRACT: LEAFY-GR time-course experiment in callus

ORGANISM(S): Arabidopsis thaliana  



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Floral induction in tissue culture: a system for the analysis of LEAFY-dependent gene regulation.

Wagner Doris D   Wellmer Frank F   Dilks Kieran K   William Dilusha D   Smith Michael R MR   Kumar Prakash P PP   Riechmann José Luis JL   Greenland Andrew J AJ   Meyerowitz Elliot M EM  

The Plant journal : for cell and molecular biology 20040701 2

We have developed a versatile floral induction system that is based on ectopic overexpression of the transcription factor LEAFY (LFY) in callus. During shoot regeneration, flowers or floral organs are formed directly from root explants without prior formation of rosette leaves. Morphological and reporter gene analyses show that leaf-like structures are converted to floral organs in response to LFY activity. Thus, increased levels of LFY activity are sufficient to bypass normal vegetative develop  ...[more]

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