Proteomics

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Targeted proteomic validation of dysregulated proteins in liver tissue of Labeo rohita after Aeromonas hydrophila infection


ABSTRACT: Aeromonas hydrophila (Ah) is an opportunistic Gram-negative bacterium and a serious global pathogen causing Motile Aeromonas Septicaemia (MAS) in fish and many other vertebrates. This study aimed at identifying the cellular proteins and processes altered by Ah infection in liver tissue of Labeo rohita, an important aquaculture species. Discovery based proteomics data could identify a panel of differentially expressed proteins (DEPs) between Control and AH condition (Ah infected group). Protein abundance changes in a few selected DEPs was then validated using Selected reaction monitoring (SRM) approach.

ORGANISM(S): Labeo Rohita

SUBMITTER: Mehar Un Nissa  

PROVIDER: PXD029483 | panorama | Tue May 02 00:00:00 BST 2023

REPOSITORIES: PanoramaPublic

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Proteomic analysis of liver tissue reveals Aeromonas hydrophila infection mediated modulation of host metabolic pathways in Labeo rohita.

Nissa Mehar Un MU   Pinto Nevil N   Ghosh Biplab B   Singh Urvi U   Goswami Mukunda M   Srivastava Sanjeeva S  

Journal of proteomics 20230310


Aeromonas hydrophila (Ah) is a Gram-negative bacterium and a serious global pathogen causing Motile Aeromonas Septicaemia (MAS) in fish leading to global loss in aquaculture. Investigation of the molecular alterations of host tissues such as liver could be a powerful approach to identify mechanistic and diagnostic immune signatures of disease pathogenesis. We performed a proteomic analysis of Labeo rohita liver tissue to examine the protein dynamics in the host cells during Ah infection. The pro  ...[more]

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