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In-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)

ABSTRACT: Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins.

ORGANISM(S): Aspergillus Fumigatus

SUBMITTER: Daniela Volke

PROVIDER: PXD045869 | panorama | Mon Apr 29 00:00:00 BST 2024

REPOSITORIES: PanoramaPublic

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Immunoproteomics enable broad identification of new <i>Aspergillus fumigatus</i> antigens in severe equine asthma.

Jentsch Maria-Christin MC Lübke Sabrina S Schrödl Wieland W Volke Daniela D Krizsan Andor A Hoffmann Ralf R Kaiser-Thom Sarah S Gerber Vinzenz V Marti Eliane E Wagner Bettina B Schnabel Christiane L CL

Frontiers in immunology 20240229

<h4>Introduction</h4>Severe equine asthma (SEA) is a common chronic disease of adult horses with characteristic recurrent airway obstruction and similarities to neutrophilic asthma in humans. As an extrinsic stimulus, hay dust exposure is a major risk factor and induces acute exacerbation in susceptible horses. However, single inducing agents of SEA have hardly been identified on a molecular basis. <i>Aspergillus fumigatus</i> (<i>A. fumigatus</i>) is a common mold species in hay and has been de ...[more]

PMID: 38487534

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