Mm_Stem_cell_dimethyl_HCD
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA155475PRJNA150567PRJNA153541PRJNA156463
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mus Musculus
SUBMITTER: Albert J. R. Heck
PROVIDER: PAe003967 | PeptideAtlas | 2012-12-31
REPOSITORIES: PeptideAtlas
Publications
The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers.
Muñoz Javier J Stange Daniel E DE Schepers Arnout G AG van de Wetering Marc M Koo Bon-Kyoung BK Itzkovitz Shalev S Volckmann Richard R Kung Kevin S KS Kung Kevin S KS Koster Jan J Radulescu Sorina S Myant Kevin K Versteeg Rogier R Sansom Owen J OJ van Es Johan H JH Barker Nick N van Oudenaarden Alexander A Mohammed Shabaz S Heck Albert J R AJ Clevers Hans H
The EMBO journal 20120612 14
Two types of stem cells are currently defined in small intestinal crypts: cycling crypt base columnar (CBC) cells and quiescent '+4' cells. Here, we combine transcriptomics with proteomics to define a definitive molecular signature for Lgr5(+) CBC cells. Transcriptional profiling of FACS-sorted Lgr5(+) stem cells and their daughters using two microarray platforms revealed an mRNA stem cell signature of 384 unique genes. Quantitative mass spectrometry on the same cell populations identified 278 p ...[more]
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