Project description:We obtained a spatial measurement of RNA and Proteins in the small intestinal epithelium along the crypt-villus axis. We found that both were spatially heterogeneous, yet often spatially anti-correlated. We developed a Bayesian approach to infer protein translation and degradation rates from the combined spatial profiles, and demonstrate that space-independent protein-synthesis delays can explain the mRNA-protein discordances. Our work provides a proteomic spatial blueprint of the Intestinal epithelium and highlights the importance of protein measurements for inferring states of tissue cells that operate outside of steady state
Project description:Receptor guanylyl cyclase C (GC-C) is expressed in the intestinal epithelia and plays a key role in intestinal physiology. Activation of GC-C by its ligands has been shown to regulate proliferation and differentiation of enterocytes along the crypt-villus axis. This study aims to identify genes regulated by GC-C activation and elevation of cGMP in colorectal carcinoma cells. This will help understand the molecular targets and biological pathways regulated by GC-C to bring about cancer cell cytostasis. ST (Cy5) Vs. Control (Cy3), ST (Cy5) Vs. Control (Cy3)
Project description:We employed a single-cell RNA sequencing approach using the 10X Genomics platform to study the diversity of telocyte populations in the mouse jejunum. Telocytes are a unique mesenchymal cell type expressing the transcription factor Foxl1 and forming a subepithelial network along the entire crypt-villus axis. This study characterizes the gene expression profiles and spatial distribution of telocytes along the crypt-villus axis of the mouse jejunum.
