Proteomics

Dataset Information

32

Hs_Hela_ArgC_CID


ABSTRACT: Human hela proteome, ArgC CID

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo sapiens  

TISSUE(S): Others

SUBMITTER: Guo X, Trudgian DC, Lemoff A, Yadavalli S, Mirzaei H 

PROVIDER: PAe005062 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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Publications

Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.

Guo Xiaofeng X   Trudgian David C DC   Lemoff Andrew A   Yadavalli Sivaramakrishna S   Mirzaei Hamid H  

Molecular & cellular proteomics : MCP 20140402 6


Bottom-up proteomics largely relies on tryptic peptides for protein identification and quantification. Tryptic digestion often provides limited coverage of protein sequence because of issues such as peptide length, ionization efficiency, and post-translational modification colocalization. Unfortunately, a region of interest in a protein, for example, because of proximity to an active site or the presence of important post-translational modifications, may not be covered by tryptic peptides. Detec  ...[more]

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