ABSTRACT: In this workspace, we have the workflow and configuration files needed to produce the human whole-body scaffold with nerves centrelines and embedded organs for the SPARC project.
Project description:In this workspace, we have the workflow and configuration files needed to produce the mouse whole-body scaffold with embedded organs for the SPARC project.
Project description:In this workspace, we have the automatic workflow and configuration files for inserting organs needed to produce the human whole-body scaffold with embedded organs for the SPARC project.
Project description:In this workspace, we have the automatic workflow and configuration files for inserting organs needed to produce the pig whole-body scaffold with embedded organs for the SPARC project.
Project description:In this workspace, we have the automatic workflow and configuration files for inserting organs needed to produce the rat whole-body scaffold with embedded organs for the SPARC project.
Project description:In this workspace we have the files needed to produce the rat whole-body scaffold with SPARC priority embedded organs for the SPARC project.
Project description:In this workspace, we have the workflow and configuration files needed to produce the 3D human whole-body scaffold with embedded organs and integrated musculoskeletal and vasculature systems for the SPARC project.
Project description:In this workspace we have the ScaffoldMaker workflow and configuration files needed to produce the human whole-body scaffold for the SPARC project.
Project description:Our knowledge about the molecular regulation of zebrafish gonad development and function is very limited. This study aims to identify gonad-expressed genes in zebrafish and to analyze their expression in various organs. Using our custom ÂGonad Uniclone Microarray v1, we performed a total of 32 hybridizations, using eight target organs with four biological replicates each. The target organs consisted of adult ovary and testis; the brain, kidney and rest-of-body (ie. whole body except the three organs listed earlier) from both male and female zebrafish, were used as controls. For simplicity, rest of body will be regarded as an organ. All targets were hybridized against a pooled common reference consisting of equal amounts of targets from all dissected organs from a single adult male and a single adult female.
Project description:In this workspace we have the Scaffold Maker workflow and configuration files needed to produce the rat whole body scaffold for the SPARC project.
Project description:For more than a century, Formalin Fixed Paraffin Embedded (FFPE) sample preparation has been the preferred method for long-term preservation of biological material. However, the use of FFPE samples for epigenomic studies has been difficult because of chromatin damage from long exposure to high concentrations of formaldehyde. Previously, we introduced Cleavage Under Targeted Accessible Chromatin (CUTAC), an antibody-targeted chromatin accessibility mapping protocol based on CUT&Tag. Here we show that simple modifications of our single-tube CUTAC protocol are sufficient to produce high-resolution maps of paused RNA Polymerase II (RNAPII) at enhancers and promoters using FFPE samples. We find that transcriptional regulatory element differences produced by FFPE-CUTAC distinguish between mouse brain tumor specimens and identify regulatory element markers with high confidence and precision. Our simple workflow is suitable for automation, making possible affordable epigenomic profiling of archived biological samples for biomarker identification, clinical applications and retrospective studies.