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Protein profiling upon miR-17-92 induction.

ABSTRACT: Methionine COFRADIC combined with Arg and Lys SILAC labeling was used to quantify proteins upon induction of miR-17-92 in SHEP neuroblastoma cells. Differently labeled cells treated with tetracycline for 72 h or left untreated, were harvested and mixed lysates were analyzed by methionine COFRADIC to isolate methionyl peptides followed by identification of these peptides by LC-MS/MS and subsequent quantification.

OTHER RELATED OMICS DATASETS IN: PRJNA127149

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neuroblastoma Cell Line

DISEASE(S): Neuroblastoma

SUBMITTER: Francis Impens

PROVIDER: PRD000321 | Pride | 2010-12-10

REPOSITORIES: Pride

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The miR-17-92 microRNA cluster regulates multiple components of the TGF-β pathway in neuroblastoma.

Mestdagh Pieter P Boström Anna-Karin AK Impens Francis F Fredlund Erik E Van Peer Gert G De Antonellis Pasqualino P von Stedingk Kristoffer K Ghesquière Bart B Schulte Stefanie S Dews Michael M Thomas-Tikhonenko Andrei A Schulte Johannes H JH Zollo Massimo M Schramm Alexander A Gevaert Kris K Axelson Håkan H Speleman Frank F Vandesompele Jo J

Molecular cell 20101201 5

The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a po ...[more]

PMID: 21145484

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