Proteomics

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Protein profiling upon miR-17-92 induction.

ABSTRACT: Methionine COFRADIC combined with Arg and Lys SILAC labeling was used to quantify proteins upon induction of miR-17-92 in SHEP neuroblastoma cells. Differently labeled cells treated with tetracycline for 72 h or left untreated, were harvested and mixed lysates were analyzed by methionine COFRADIC to isolate methionyl peptides followed by identification of these peptides by LC-MS/MS and subsequent quantification.

OTHER RELATED OMICS DATASETS IN: PRJNA127149

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neuroblastoma Cell Line

DISEASE(S): Neuroblastoma

SUBMITTER: Francis Impens  

PROVIDER: PRD000321 | Pride | 2010-12-10

REPOSITORIES: Pride

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PRIDE_Exp_Complete_Ac_14860.pride.mgf.gz Mgf
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Publications

The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a po  ...[more]

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