Protein profiling upon miR-17-92 induction.

ABSTRACT: Methionine COFRADIC combined with Arg and Lys SILAC labeling was used to quantify proteins upon induction of miR-17-92 in SHEP neuroblastoma cells. Differently labeled cells treated with tetracycline for 72 h or left untreated, were harvested and mixed lysates were analyzed by methionine COFRADIC to isolate methionyl peptides followed by identification of these peptides by LC-MS/MS and subsequent quantification.

OTHER RELATED OMICS DATASETS IN: PRJNA127149

INSTRUMENT(S): LTQ-Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neuroblastoma Cell Line

DISEASE(S): Neuroblastoma

SUBMITTER: Francis Impens  

LAB HEAD: Francis Impens

PROVIDER: PRD000321 | Pride | 2010-12-10

REPOSITORIES: Pride