Name: A shotgun proteomics reference map for the alkali-sensitive covalently-linked proteins of the cell wall of A. fumigatus during specific stages of early fungal development (dormant conidia, isotropically expanding conidia, early germ tube emergence and young hyphae).
Keywords: Not available

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A shotgun proteomics reference map for the alkali-sensitive covalently-linked proteins of the cell wall of A. fumigatus during specific stages of early fungal development (dormant conidia, isotropically expanding conidia, early germ tube emergence and young hyphae).

ABSTRACT: Four different developmental time points representing the Afu development from dormant conidia into young hyphae (0h, 4h, 6h, 8h) were chosen. Cell walls of the Afu samples were extracted using denaturing methods followed by isolation of solubilized proteins. Samples were processed using filter aided sample preparation (FASP) / trypsin digestion. The peptides were analyzed by one-dimensional LC (capillary C18 nano-Eflow mode) on-line with a nano-ESI source and tandem MS (LTQ ion trap).

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Aspergillus Fumigatus Af293

SUBMITTER: John Braisted

LAB HEAD: John Braisted

PROVIDER: PRD000567 | Pride | 2016-12-20

REPOSITORIES: Pride

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Development stage-specific proteomic profiling uncovers small, lineage specific proteins most abundant in the Aspergillus Fumigatus conidial proteome.

Suh Moo-Jin MJ Fedorova Natalie D ND Cagas Steven E SE Hastings Susan S Fleischmann Robert D RD Peterson Scott N SN Perlin David S DS Nierman William C WC Pieper Rembert R Momany Michelle M

Proteome science 20120430 1

<h4>Background</h4>The pathogenic mold Aspergillus fumigatus is the most frequent infectious cause of death in severely immunocompromised individuals such as leukemia and bone marrow transplant patients. Germination of inhaled conidia (asexual spores) in the host is critical for the initiation of infection, but little is known about the underlying mechanisms of this process.<h4>Results</h4>To gain insights into early germination events and facilitate the identification of potential stage-specifi ...[more]

PMID: 22545825

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