Proteomics

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Determination of amino acid sequence of a common dentex (Dentex dentex) egg yolk vitellogenin B derived protein (17.69 kDa) using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

ABSTRACT: The 17.62 kDa vitellogenin B derived yolk protein was purified by a specific cation exchange chromatography, subjected to a liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and finally identified using short MS/MS protein signatures similar to vitellogenin of a near species from the same family, red seabream, Pagrus major, (Temminck and Schlegel, 1843).

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Dentex Dentex (common Dentex)

TISSUE(S): Egg

SUBMITTER: Seyed-Mohammadreza SAMAEE  

PROVIDER: PRD000817 | Pride | 2010-02-12

REPOSITORIES: Pride

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Publications

Quantitative composition of vitellogenin-derived yolk proteins and their effects on viability of embryos and larvae of common dentex (Dentex dentex), a marine pelagophil teleost.

Samaee Seyed-Mohammadreza SM   Lahnsteiner Franz F   Giménez Gemma G   Estévez Alicia A   Sarg Bettina B   Lindner Herbert H  

Journal of experimental zoology. Part A, Ecological genetics and physiology 20090801 7

To evaluate the importance of the quantitative composition of vitellogenin (Vtg)-derived egg yolk proteins during initial events of Dentex dentex life, viable eggs were obtained from captive broodstocks. Viability parameters (Vps; egg buoyancy (FR), hatching rate (HR), and larval survival rate (SR) at 0, 3, and 5 days posthatch (dph)) were determined for 70 egg batches. Vtg-derived yolk proteins were purified from the eggs using cation-exchange chromatography, separated and detected by SDS-PAGE,  ...[more]

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