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Quantitative proteome profiling of Caenorhabditis elegans pept-1(lg601) and N2(Bristol) during ontogenesis.

ABSTRACT: PEPT-1 is responsible for the uptake of di-and tripeptides form the intestinal lumen into the epithelial cells. Knock-out of pept-1 in C.elegans results in a severe phenotype. Despite a reduction of brood size and a retarded development, pept-1(lg601) depicts increased stress resistance. Therefore, populations of pept1(lg601) as well as N2(Bristol) were synchronized and sampled at various time points during development. Applying 15N metabolic labeling in combination with shotgun LC/MS-MS, we determined the proteome of both strains during development.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Gerhard Mayer

LAB HEAD: Prof. Hannelore Daniel

PROVIDER: PXD000310 | Pride | 2016-07-15

REPOSITORIES: Pride

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Intestinal amino acid availability via PEPT-1 affects TORC1/2 signaling and the unfolded protein response.

Geillinger Kerstin E KE Kuhlmann Katja K Eisenacher Martin M Giesbertz Pieter P Meyer Helmut E HE Daniel Hannelore H Spanier Britta B

Journal of proteome research 20140716 8

The intestinal peptide transporter PEPT-1 plays an important role in development, growth, reproduction, and stress tolerance in Caenorhabditis elegans, as revealed by the severe phenotype of the pept-1-deficient strain. The reduced number of offspring and increased stress resistance were shown to result from changes in the insulin/IGF-signaling cascade. To further elucidate the regulatory network behind the phenotypic alterations in PEPT1-deficient animals, a quantitative proteome analysis combi ...[more]

PMID: 24999909

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