A multi-omic analysis of an Enterococcus faecium mutant reveals genetic mutation and dramatic changes of mRNA and protein expressions

ABSTRACT: Three iTRAQ8plex experiment replicate using TripleToF 5600 system. Protein identification were performed using the ProteinPilot software (version 4.0.8085,Paragon Algorithm version 4.0.0.0).All MS/MS spectra was search against amino acids sequences which is annotated from genome. The search parameters was set as follows: Cys. Alkylation: Iodoacetic acid, Digestion: Trypsin, ID Focus: Biological modifications, Search Effort: Thorough. The search results was filter using Local FDR 5%.

INSTRUMENT(S):

ORGANISM(S): Enterococcus Faecalis (streptococcus Faecalis)

SUBMITTER: Liu liu  

LAB HEAD: Liu liu

PROVIDER: PXD000326 | Pride | 2014-04-16

REPOSITORIES: Pride