Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration

ABSTRACT: 3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed by further enrichment by IMAC. Peptides were anaylzed on an Orbitrap Elite with CID and HCD scans for MS/MS peptide identification.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Blood

SUBMITTER: Hannah Johnson  

LAB HEAD: Hannah Johnson

PROVIDER: PXD000440 | Pride | 2013-11-07

REPOSITORIES: Pride