Identification of SUMO sites by LC-MSMS

ABSTRACT: We developed a novel method for the identification of SUMO sites by expression of His-tagged SUMO mutants and affinity purification of SUMOylated proteins, followed by trypsin digestion and immunocapture of peptides containing diglycine signature tags. Lab Head: Dr Pascale Cossart, [e-mail] Institut Pasteur Unité des Interactions Bactéries-Cellules, Inserm U604, INRA USC2020 25 rue du Dr. Roux 75015 Paris France

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Francis Impens  

LAB HEAD: Francis Impens

PROVIDER: PXD000459 | Pride | 2014-07-29

REPOSITORIES: Pride