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Yersinia enterocolitica SOR17 - The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica

ABSTRACT: We work with the bacterium Yersinia enterocolitica, a gastrointestinal pathogen. In this study we characterized a bacterial mutant strain called SOR17. We compared the protein extracts of SOR17 with that of the parental strain JB580v. Bacteria were grown in triplicate for 5 h at 37°C, and following bacterial disruption by French press, proteins were fractionated into soluble or membrane proteins. We also compared total protein extracts of bacteria grown for 16 h at 27°C. Two-dimensional electrophoresis were performed and proteins were stained with Coomassie brilliant blue. Spots whose abundance changed more than 2 fold were excised and sent to mass spectrometry for identification by either MALDI-TOF PMF or LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Yersinia Enterocolitica Bacteria

SUBMITTER: Ombeline Rossier

PROVIDER: PXD000475 | Pride | 2014-04-16

REPOSITORIES: Pride

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The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica.

Kakoschke Tamara T Kakoschke Sara S Magistro Giuseppe G Schubert Sören S Borath Marc M Heesemann Jürgen J Rossier Ombeline O

PloS one 20140115 1

To adapt to changes in environmental conditions, bacteria regulate their gene expression at the transcriptional but also at the post-transcriptional level, e.g. by small RNAs (sRNAs) which modulate mRNA stability and translation. The conserved RNA chaperone Hfq mediates the interaction of many sRNAs with their target mRNAs, thereby playing a global role in fine-tuning protein production. In this study, we investigated the significance of Hfq for the enteropathogen Yersina enterocolitica serotype ...[more]

PMID: 24454955

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