Dataset Information
Cell-Specific Labeling Enzymes For Analysis of Cell-Cell Communication in Continuous Co-Culture
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Christopher Tape
LAB HEAD: Claus Jorgensen
PROVIDER: PXD000739 | Pride | 2022-02-22
REPOSITORIES: Pride
Publications
Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture.
Molecular & cellular proteomics : MCP 20140512 7
We report the orthologous screening, engineering, and optimization of amino acid conversion enzymes for cell-specific proteomic labeling. Intracellular endoplasmic-reticulum-anchored Mycobacterium tuberculosis diaminopimelate decarboxylase (DDC(M.tub-KDEL)) confers cell-specific meso-2,6-diaminopimelate-dependent proliferation to multiple eukaryotic cell types. Optimized lysine racemase (Lyr(M37-KDEL)) supports D-lysine specific proliferation and efficient cell-specific isotopic labeling. When e ...[more]
