Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Plasma
SUBMITTER: Ya Jin
LAB HEAD: Ya Jin
PROVIDER: PXD000852 | Pride | 2014-04-07
REPOSITORIES: Pride
Jin Ya Y Bu Shujie S Zhang Jun J Yuan Qi Q Manabe Takashi T Tan Wen W
Electrophoresis 20140519 14
A human plasma sample was subjected to nondenaturing micro 2DE and a gel area (5 mm × 18 mm) that includes high-density lipoprotein (HDL) was cut into 1 mm × 1 mm squares, then the proteins in the 90 gel pieces were analyzed by quantitative LC-MS/MS. Grid-cutting of the gel was employed to; (i) ensure the total analysis of the proteins in the area, (ii) standardize the conditions of analysis by LC-MS/MS, (iii) reconstruct the protein distribution patterns from the quantity data. Totally 154 prot ...[more]