Proteomics

Dataset Information

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Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions


ABSTRACT: We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of the sRNAs was down-regulated in cells grown in poor medium with glucose as the sole carbon source but not without lrp, indicating that sRNA expression is controlled by the stringent response. N. meningitidis, over-expressing the sRNAs replicated in blood, but not in human cerebrospinal fluid. In addition, Lrp synthesis was inhibited by direct interaction between the sRNA and the 5’ UTR of lrp. sRNAs control adaptation of N. meningitidis to variation in nutrient supply in different niches of its host.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Neisseria meningitidis serogroup B  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Meningococcal Meningitis

SUBMITTER: Gertjan Kramer  

LAB HEAD: Dave Speijer

PROVIDER: PXD000891 | Pride | 2017-03-22

REPOSITORIES: pride

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Publications

Neisseria meningitidis Uses Sibling Small Regulatory RNAs To Switch from Cataplerotic to Anaplerotic Metabolism.

Pannekoek Yvonne Y   Huis In 't Veld Robert A G RA   Schipper Kim K   Bovenkerk Sandra S   Kramer Gertjan G   Brouwer Matthijs C MC   van de Beek Diederik D   Speijer Dave D   van der Ende Arie A  

mBio 20170321 2


Neisseria meningitidis (the meningococcus) is primarily a commensal of the human oropharynx that sporadically causes septicemia and meningitis. Meningococci adapt to diverse local host conditions differing in nutrient supply, like the nasopharynx, blood, and cerebrospinal fluid, by changing metabolism and protein repertoire. However, regulatory transcription factors and two-component systems in meningococci involved in adaptation to local nutrient variations are limited. We identified novel sibl  ...[more]

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