Neisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions

ABSTRACT: We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of the sRNAs was down-regulated in cells grown in poor medium with glucose as the sole carbon source but not without lrp, indicating that sRNA expression is controlled by the stringent response. N. meningitidis, over-expressing the sRNAs replicated in blood, but not in human cerebrospinal fluid. In addition, Lrp synthesis was inhibited by direct interaction between the sRNA and the 5’ UTR of lrp. sRNAs control adaptation of N. meningitidis to variation in nutrient supply in different niches of its host.

INSTRUMENT(S):

ORGANISM(S): Neisseria Meningitidis Serogroup B

DISEASE(S): Meningococcal Meningitis

SUBMITTER: Gertjan Kramer  

LAB HEAD: Dave Speijer

PROVIDER: PXD000891 | Pride | 2017-03-22

REPOSITORIES: Pride