Method to identify novel proteoform peptides from salivary supernatant.

ABSTRACT: We describe the use Galaxy framework to facilitate complete proteogenomic analysis for a representative salivary dataset. We demonstrate how Galaxy’s many features make it a unique and ideal solution for proteogenomic analysis. We highlight Galaxy’s flexibility by creating a modular workflow incorporating both established and customized software and processing steps that improve depth and quality of proteogenomic results. We demonstrate Galaxy’s accessibility, via the easy sharing of complete, and even complex (approximately 140 steps), proteogenomic workflows, which can be used and customized by others via a public instance of the framework (usegalaxyp.org). Our results provide a blueprint for the establishment of the Galaxy framework as an ideal solution for the emerging field of proteogenomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Disease Free

SUBMITTER: Pratik Jagtap  

LAB HEAD: Timothy Griffin

PROVIDER: PXD000991 | Pride | 2015-01-05

REPOSITORIES: Pride