Proteomics

Dataset Information

200

Profiling the proteome of Mycobacterium tuberculosis during dormancy and reactivation


ABSTRACT: Tuberculosis, caused by Mycobacterium tuberculosis, still remains a major global health problem. The main obstacle in eradicating this disease is the ability of this pathogen to remain dormant in macrophages, and to get reactivated later under immuno-compromised conditions. The physiology of hypoxic nonreplicating M. tuberculosis is well studied using many in vitro dormancy models. However, the physiological changes that take place during the shift from dormancy to aerobic growth (reactivation) have rarely been subjected to a detailed investigation. In this study, we developed an in vitro reactivation system by re-aerating bacteria that were made dormant employing Wayne’s dormancy model, and compared the proteome profiles of dormant and reactivated bacteria using label-free one-dimensional LC/MS/MS analysis.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Mycobacterium tuberculosis H37Rv  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Tuberculosis

SUBMITTER: Ajay Kumar  

LAB HEAD: R. Ajay Kumar

PROVIDER: PXD001158 | Pride | 2015-06-01

REPOSITORIES: Pride

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Publications

Profiling the Proteome of Mycobacterium tuberculosis during Dormancy and Reactivation.

Gopinath Vipin V   Raghunandanan Sajith S   Gomez Roshna Lawrence RL   Jose Leny L   Surendran Arun A   Ramachandran Ranjit R   Pushparajan Akhil Raj AR   Mundayoor Sathish S   Jaleel Abdul A   Kumar Ramakrishnan Ajay RA  

Molecular & cellular proteomics : MCP 20150529 8


Tuberculosis, caused by Mycobacterium tuberculosis, still remains a major global health problem. The main obstacle in eradicating this disease is the ability of this pathogen to remain dormant in macrophages, and then reactivate later under immuno-compromised conditions. The physiology of hypoxic nonreplicating M. tuberculosis is well-studied using many in vitro dormancy models. However, the physiological changes that take place during the shift from dormancy to aerobic growth (reactivation) hav  ...[more]

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