Dataset Information

Https://edelivery.oracle.com/akam/otn/java/jdk/8u202-b08/1961070e4c9b4e26a04e7f5a083f551e/jre-8u202-windows-i586-iftw.exe

ABSTRACT: This study examines how aging influences the proteomic landscape of human spermatozoa. Proteomic analysis was done on sperm samples from three groups of normal, fertile men: young adults (21–30 years), late adults (31–40 years), and advanced age (41–51 years). The analysis revealed dynamic age-related alterations in human sperm proteins potentially impacting key sperm physiological functions such as motility, metabolism, and fertilization.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis, Sperm

DISEASE(S): Infertility

SUBMITTER: Ayodele Alaiya

LAB HEAD: Dr. Ayodele Alaiya

PROVIDER: PXD062650 | Pride | 2025-07-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ALL-QUANT-IDs-467-ALL-GRPS.csv	Csv
	EXPORT-PEPTIDE-3D-SPECTRAL-TO-MGF.mgf	Mgf
	EXPORT_SPECTRAL_LIBRARIES.msp	Other
	P1-21-30--S-NS-28062022-01.raw.rar	Raw
	P1-21-30--S-NS-28062022-02.raw.zip	Raw

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Publications

Age-Associated Proteomic Changes in Human Spermatozoa.

Beg Mohd Amin MA Ismail Abrar Osama AO Alaiya Ayodele A Khan Firdous Ahmad FA Hamoda Taha Abo-Almagd Abdel-Meguid TAA Sheikh Ishfaq Ahmad IA Sharma Priyanka P Baothman Omar Mohammed OM Alkhzaim Ali Hasan AH Shinwari Zakia Z Abuzinadah Rinad Fahad RF Mohammed Arif A Assiri Abdullah Mohammed AM Abuzenadah Adel Mohammad AM Memili Erdogan E Feugang Jean Magloire JM

International journal of molecular sciences 20250625 13

Advancing age in men significantly contributes to declining sperm fertility. Information on age-related proteomic changes in spermatozoa is limited. This study involved normal fertile Arab men in three age groups: young adult (21-30 years; n = 6), late adult (31-40 years; n = 7), and advanced age (40-51 years; n = 5). Gradient-purified spermatozoa were analyzed using LC-MS/MS and proteomic data were processed using Progenesis QI (QIfp) v3.0 and UniProt/SwissProt. Significant ...[more]

PMID: 40649876

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Project description:Infertility is a widespread problem, affecting around 15% of couples worldwide, and is defined as the inability to achieve pregnancy within one year despite unprotected intercourse 1. Infertility can be caused by either male or female reproductive issues. Various medical conditions including malignancies, infections, urogenital conditions, or genetic causes can contribute to male infertility. However, 30-40% of men in their reproductive age are affected by idiopathic infertility, according to the guidelines of European Association of Urology (EAU) 1. Towards a better understanding of male infertility, it is mandatory to achieve a comprehensive understanding of involved genes, their RNA transcripts, and regulatory factors, including miRNAs, which influence the expression level of proteins. Therefore, such proteins need to be identified to investigate their role in spermatogenesis and male infertility. Although there are numerous studies on RNAs, including miRNAs related to male infertility 2-9, there are few studies aiming to cover the whole proteome of human sperm 10. The sperm transcriptome comprises a total of 60,505 transcripts including 11,688 differentially expressed transcripts in infertile and fertile men, as reported by Joshi et al. (2022)11. The entire sperm proteome encompasses 6871 proteins, as summarized by Castillo et al. (2018)10. Nevertheless, there is still a lack of high-throughput studies aiming to identify dysregulated proteins in sperm from subfertile men. Only few studies focused on comparisons of the sperm proteome in men with asthenozoospermia and there is virtually no proteomic studies of oligoasthenozoospermic men 12. Some identified proteins in sperm have functions in maintaining sperm motility and enabling fertilization and are involved in structural composition and/or energy metabolism 12-14 and others are not yet functionally characterized. In this study, we employed Mass spectrometry (MS) technology that is still rarely used in the field of human reproductive research to investigate the proteomic landscape of human sperm and their differential expression patterns in men with subfertility.