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Diethylation

ABSTRACT: In this study we compared diethylation using acetaldehyde-13C2/13C1 and acetaldehyde-2H4/1H4. Light and heavy labeled samples were combined in a ratio of 1:1 and analyzed on a LC-MSMS system using an OrbitrapXL. We analyzed ten techical replicates of each labeling version (13C/12C and 2H/1H) and compared them in regards of labeling yield, quantification accuracy and precision.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Christian Koehler

LAB HEAD: Dr. Bernd Thiede

PROVIDER: PXD001256 | Pride | 2022-03-02

REPOSITORIES: Pride

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Publications

The impact of carbon-13 and deuterium on relative quantification of proteins using stable isotope diethyl labeling.

Koehler Christian J CJ Arntzen Magnus Ø MØ Thiede Bernd B

Rapid communications in mass spectrometry : RCM 20150501 9

<h4>Rationale</h4>Stable isotopic labeling techniques are useful for quantitative proteomics. A cost-effective and convenient method for diethylation by reductive amination was established. The impact using either carbon-13 or deuterium on quantification accuracy and precision was investigated using diethylation.<h4>Methods</h4>We established an effective approach for stable isotope labeling by diethylation of amino groups of peptides. The approach was validated using matrix-assisted laser desor ...[more]

PMID: 26377011

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