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Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7

ABSTRACT: Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterisation of phagosomes from RAW 264.7 and bone marrow-derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicates that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Matthias Trost

LAB HEAD: Matthias Trost

PROVIDER: PXD001293 | Pride | 2015-02-10

REPOSITORIES: Pride

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High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.

Guo Manman M Härtlova Anetta A Dill Brian D BD Prescott Alan R AR Gierliński Marek M Trost Matthias M

Proteomics 20150205 18

Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal prote ...[more]

PMID: 25504905

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Temporal and ligand effects on phagosome maturation

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Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7

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Effect of LPS and LPS-binding protein treatment for 8 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 8 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 8 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

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