Proteomics

Dataset Information

126

Exosomal proteome from mouse CSF


ABSTRACT: Exosomes were isolated from the mouse CSF using total exosome isolation kit (Life Technologies) from 0h and 6h LPS treated mice. These exosomes were separated on the RP-HPLC in to 20 fractions and analysed on the Qexactive.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus musculus  

TISSUE(S): Cerebrospinal Fluid

DISEASE(S): Not Available

SUBMITTER: Sriram Balusu  

PROVIDER: PXD001346 | Pride | 2016-09-27

REPOSITORIES: Pride

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Publications

Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles.

Balusu Sriram S   Van Wonterghem Elien E   De Rycke Riet R   Raemdonck Koen K   Stremersch Stephan S   Gevaert Kris K   Brkic Marjana M   Demeestere Delphine D   Vanhooren Valerie V   Hendrix An A   Libert Claude C   Vandenbroucke Roosmarijn E RE  

EMBO molecular medicine 20161004 10


Here, we identified release of extracellular vesicles (EVs) by the choroid plexus epithelium (CPE) as a new mechanism of blood-brain communication. Systemic inflammation induced an increase in EVs and associated pro-inflammatory miRNAs, including miR-146a and miR-155, in the CSF Interestingly, this was associated with an increase in amount of multivesicular bodies (MVBs) and exosomes per MVB in the CPE cells. Additionally, we could mimic this using LPS-stimulated primary CPE cells and choroid pl  ...[more]

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