Proteome analysis of NGF stimulated PC12 cells

ABSTRACT: We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced changes in protein abundance using the rat PC12 cell line. Cells were stimulated with NGF for 0, 24 and 48 h in a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10). All experiments were performed as biological replicates.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Adrenal Medulla Chromaffin Cell, Adrenal Medulla

DISEASE(S): Adrenal Gland Pheochromocytoma

SUBMITTER: Kristina Bennet Emdal  

LAB HEAD: Jesper Velgaard Olsen

PROVIDER: PXD001478 | Pride | 2015-05-06

REPOSITORIES: Pride