Proteomics

Dataset Information

70

Proteome Analysis of Actinoplanes sp. SE50/110


ABSTRACT: The acarviose metabolite acarbose is an a glucosidase inhibitor produced by Actinoplanes sp. SE50/110. It is medically important because it is used in the treatment of type 2 diabetes. In this work a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out. The associated txt and RAW files were used for two different analyses and publications. While one study focused on a comparative analysis of Actinoplanes sp. SE50/110 to elucidate differences in the proteome cultures that were grown with either maltose or glucose, the other study applied spectral counting and analyzed only the maltose-grown cultures to determine the major proteins and their location in the cell. The txt files for the comparative data are labeled as "heavy_light" and of the spectral counting data as "light". Both datasets were derived from the same RAW files.

REANALYSED by: GPM32320019694

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Actinoplanes sp. SE50/110  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Andreas Otto  

LAB HEAD: Andreas Otto

PROVIDER: PXD001497 | Pride | 2015-10-21

REPOSITORIES: Pride

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Publications

Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters.

Wendler Sergej S   Otto Andreas A   Ortseifen Vera V   Bonn Florian F   Neshat Armin A   Schneiker-Bekel Susanne S   Wolf Timo T   Zemke Till T   Wehmeier Udo F UF   Hecker Michael M   Kalinowski Jörn J   Becher Dörte D   Pühler Alfred A  

Journal of proteomics 20151025


Actinoplanes sp. SE50/110 is known for the production of the α-glucosidase inhibitor and anti-diabetic drug acarbose. Acarbose (acarviosyl-maltose) is produced as the major product when the bacterium is grown in medium with maltose, while acarviosyl-glucose is the major product when glucose is the sole carbon source in the medium. In this study, a state-of-the-art proteomics approach was applied combining subcellular fractionation, in vivo metabolic labeling and shotgun mass spectrometry to anal  ...[more]

Publication: 1/2

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