Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger

ABSTRACT: Background: HacA/Xbp1 is a conserved bZIP transcription factor in eukaryotic cells which regulates gene expression in response to various forms of secretion stress and as part of secretory cell differentiation. In the present study, we replaced the endogenous hacA gene of an Aspergillus niger strain with a gene encoding a constitutively active form of the HacA transcription factor (HacACA). The impact of constitutive HacA activity during exponential growth was explored in bioreactor controlled cultures using transcriptomic analysis to identify affected genes and processes. Results: Transcription profiles for the wild-type strain (HacAWT) and the HacACA strain were obtained using Affymetrix GeneChip analysis of three replicate batch cultures of each strain. In addition to the well known Ha

ORGANISM(S): Aspergillus niger

SUBMITTER: Benjamin Nitsche 

PROVIDER: E-GEOD-39070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress