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Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs

ABSTRACT: We used here high-throughput quantitative proteomics and targeted mRNA quantification to estimate in human cell cultures the mass and copy numbers of the most abundant proteins that become significantly accumulated, depleted or unchanged during and following a 4h heat shock at 41C.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell

SUBMITTER: Manfredo Quadroni

LAB HEAD: Pierre Goloubinoff

PROVIDER: PXD001666 | Pride | 2015-04-10

REPOSITORIES: Pride

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Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs.

Finka Andrija A Sood Vishal V Quadroni Manfredo M Rios Paolo De Los Pde L Goloubinoff Pierre P

Cell stress & chaperones 20150408 4

Classic semiquantitative proteomic methods have shown that all organisms respond to a mild heat shock by an apparent massive accumulation of a small set of proteins, named heat-shock proteins (HSPs) and a concomitant slowing down in the synthesis of the other proteins. Yet unexplained, the increased levels of HSP messenger RNAs (mRNAs) may exceed 100 times the ensuing relative levels of HSP proteins. We used here high-throughput quantitative proteomics and targeted mRNA quantification to estimat ...[more]

PMID: 25847399

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