Proteomics

Dataset Information

94

Quantitative Phosphoproteomic Identification of Novel Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells


ABSTRACT: The kidney distal convoluted tubule (DCT) plays an important role in body sodium regulation and thus control of blood pressure. The main sodium reabsorption pathways in the DCT are the epithelial sodium channel (ENaC) and the thiazide-sensitive NaCl cotransporter (NCC), the functions of which can be modulated by the hormone vasopressin (VP) acting via uncharacterized signaling cascades. We performed large scale stable isotope labeling by amino acids in cell culture (SILAC) based quantitative phosphoproteomics of cultured mouse DCT cells (mpkDCT) to map global changes in protein phosphorylation events upon acute treatment with the VP type II receptor agonist 1-desamino-8-D-arginine vasopressin (dDAVP). The aim of this study is to identify unique VP signaling cascades in DCT cells that may be important for regulating ENaC or NCC activity. These pathways may be novel targets for modulation of blood pressure.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Kidney

DISEASE(S): Not Available

SUBMITTER: Qi Wu  

LAB HEAD: Robert A. Fenton

PROVIDER: PXD001729 | Pride | 2015-08-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
S1_dDAVP_phos.msf Msf
S1_dDAVP_phos_F1_01.raw Raw
S1_dDAVP_phos_F1_02.raw Raw
S1_dDAVP_phos_F1_03.raw Raw
S1_dDAVP_phos_F2_01.raw Raw
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Publications

A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells.

Cheng Lei L   Wu Qi Q   Kortenoeven Marleen L A MLA   Pisitkun Trairak T   Fenton Robert A RA  

Scientific reports 20150804


The kidney distal convoluted tubule (DCT) plays an essential role in maintaining body sodium balance and blood pressure. The major sodium reabsorption pathway in the DCT is the thiazide-sensitive NaCl cotransporter (NCC), whose functions can be modulated by the hormone vasopressin (VP) acting via uncharacterized signaling cascades. Here we use a systems biology approach centered on stable isotope labeling by amino acids in cell culture (SILAC) based quantitative phosphoproteomics of cultured mou  ...[more]

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