Proteomics

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Mesenchymal stem cell integrin-associated complexes

ABSTRACT: The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.

INSTRUMENT(S): LTQ Velos, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Stem Cell, Cell Culture

SUBMITTER: Jon Humphries  

LAB HEAD: Martin J Humphries

PROVIDER: PXD001809 | Pride | 2015-07-20

REPOSITORIES: Pride

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Proteomic analysis of integrin-associated complexes from mesenchymal stem cells.

Ajeian Jila N JN   Horton Edward R ER   Astudillo Pablo P   Byron Adam A   Askari Janet A JA   Millon-Frémillon Angélique A   Knight David D   Kimber Susan J SJ   Humphries Martin J MJ   Humphries Jonathan D JD  

Proteomics. Clinical applications 20150917 1

<h4>Purpose</h4>Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmi  ...[more]

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