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Integrin adhesion complex (IAC) analysis from human pancreatic ductal cells (HPDE)

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is characterised by an abundant desmoplastic reaction which includes an excess production of extracellular matrix and interacts with integrin adhesion receptors. Integrin adhesion complexes formed by HPDE cells (H6c7), with and without expression of mutant KRas G12V, were isolated and analysed by LC-MSMS

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Pancreatic Ductal Carcinoma

SUBMITTER: Jon Humphries

LAB HEAD: Martin J Humphries

PROVIDER: PXD027827 | Pride | 2022-05-20

REPOSITORIES: Pride

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Pancreatic ductal adenocarcinoma cells employ integrin α6β4 to form hemidesmosomes and regulate cell proliferation.

Humphries Jonathan D JD Zha Junzhe J Burns Jessica J Askari Janet A JA Below Christopher R CR Chastney Megan R MR Jones Matthew C MC Mironov Aleksandr A Knight David D O'Reilly Derek A DA Dunne Mark J MJ Garrod David R DR Jorgensen Claus C Humphries Martin J MJ

Matrix biology : journal of the International Society for Matrix Biology 20220409

Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis due to its aggressive progression, late detection and lack of druggable driver mutations, which often combine to result in unsuitability for surgical intervention. Together with activating mutations of the small GTPase KRas, which are found in over 90% of PDAC tumours, a contributory factor for PDAC tumour progression is formation of a rigid extracellular matrix (ECM) and associated desmoplasia. This response leads to aberrant integr ...[more]

PMID: 35405272

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