Proteomics,Multiomics

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Proteomics analysis of the effect of deletion of PUF3 on yeast via LC-MSMS.

ABSTRACT: The Puf family of RNA-binding proteins regulate the expression of genes by controlling protein synthesis or stimulating RNA decay. Puf3p is one of six Puf-family members in Saccharomyces cerevisiae. Puf3p has been characterised previously as regulating mRNA decay of nuclear mRNAs that encode for mitochondrial proteins. We undertook a series of genome wide approaches to identify an expanded set of Puf3p target mRNAs and to quantitatively assess the impact of loss of PUF3 on the expression control in actively growing wild type and puf3Δ strains. Here we report our quantifications of relative protein levels. The data suggest that loss of Puf3p only impacts on a small proportion of the proteins encoded by its mRNA targets.

INSTRUMENT(S): Progenesis LC-MS, Dionex instrument model, Mascot, LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: David Talavera  

LAB HEAD: Graham Pavitt

PROVIDER: PXD001903 | Pride | 2016-04-13

REPOSITORIES: Pride

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Publications

Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.

Kershaw Christopher J CJ   Costello Joseph L JL   Talavera David D   Rowe William W   Castelli Lydia M LM   Sims Paul F G PF   Grant Christopher M CM   Ashe Mark P MP   Hubbard Simon J SJ   Pavitt Graham D GD  

Scientific reports 20151023

The PUF family of RNA-binding proteins regulate gene expression post-transcriptionally. Saccharomyces cerevisiae Puf3p is characterised as binding nuclear-encoded mRNAs specifying mitochondrial proteins. Extensive studies of its regulation of COX17 demonstrate its role in mRNA decay. Using integrated genome-wide approaches we define an expanded set of Puf3p target mRNAs and quantitatively assessed the global impact of loss of PUF3 on gene expression using mRNA and polysome profiling and quantita  ...[more]

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