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Human HeLa SILAC LC MS/MS - Hypoxia Strongly Affects Mitochondrial Ribosomal Proteins and Translocases, as Shown by Quantitative Proteomics of HeLa Cells

ABSTRACT: Human HeLa cells were either kept in a hypoxic environment or under normoxic conditions. 137 proteins were found to be regulated, with maximum alteration of 18-fold. In particular, three clusters of differentially regulated proteins were identified, showing significant up-regulation of glycolysis and down-regulation of mitochondrial ribosomal proteins and translocases. This interaction is likely orchestrated by HIF-1. We also investigated the effect of hypoxia on the cell cycle, which is arrested at a restriction point in G1, and shows a prolonged S phase under these conditions.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Christian Koehler

LAB HEAD: Bernd Thiede

PROVIDER: PXD002001 | Pride | 2017-01-02

REPOSITORIES: Pride

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Hypoxia Strongly Affects Mitochondrial Ribosomal Proteins and Translocases, as Shown by Quantitative Proteomics of HeLa Cells.

Bousquet Paula A PA Sandvik Joe Alexander JA Arntzen Magnus Ø MØ Jeppesen Edin Nina F NF Christoffersen Stine S Krengel Ute U Pettersen Erik O EO Thiede Bernd B

International journal of proteomics 20150902

Hypoxia is an important and common characteristic of many human tumors. It is a challenge clinically due to the correlation with poor prognosis and resistance to radiation and chemotherapy. Understanding the biochemical response to hypoxia would facilitate the development of novel therapeutics for cancer treatment. Here, we investigate alterations in gene expression in response to hypoxia by quantitative proteome analysis using stable isotope labeling with amino acids in cell culture (SILAC) in ...[more]

PMID: 26421188

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