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Changes in proteome upon mTOR activation

ABSTRACT: mTOR activation has been known to affect protein synthesis. To identify molecular signatures in transcriptome that could enhance protein synthesis, RNA-seq and quantitative proteomics studies were conducted using WT and TSC1 null MEFs. In this study, we found that the activation of mTOR leads to genome-wide 3'UTR shortening in mRNAs by alternative polyadenylation and activates ubiquitin-mediated proteolysis. The accession number for the RNA-seq data in this study is SRP056624.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Ebbing de Jong

LAB HEAD: Jeongsik Yong

PROVIDER: PXD002006 | Pride | 2015-06-16

REPOSITORIES: Pride

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mRNA 3'-UTR shortening is a molecular signature of mTORC1 activation.

Chang Jae-Woong JW Zhang Wei W Yeh Hsin-Sung HS de Jong Ebbing P EP Jun Semo S Kim Kwan-Hyun KH Bae Sun S SS Beckman Kenneth K Hwang Tae Hyun TH Kim Kye-Seong KS Kim Do-Hyung DH Griffin Timothy J TJ Kuang Rui R Yong Jeongsik J

Nature communications 20150615

Mammalian target of rapamycin (mTOR) enhances translation from a subset of messenger RNAs containing distinct 5'-untranslated region (UTR) sequence features. Here we identify 3'-UTR shortening of mRNAs as an additional molecular signature of mTOR activation and show that 3'-UTR shortening enhances the translation of specific mRNAs. Using genetic or chemical modulations of mTOR activity in cells or mouse tissues, we show that cellular mTOR activity is crucial for 3'-UTR shortening. Although long ...[more]

PMID: 26074333

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