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Expression dynamics in mammalian cells responding to protein misfolding stress

ABSTRACT: The relative importance of gene expression regulation at the mRNA versus the protein-level is a matter of ongoing debate. We present an in-depth analysis of a cervical cancer cell line responding to protein misfolding stress induced by dithiothreitol treatment, quantifying the dynamics of mRNA and protein concentrations for >3,500 genes over a time-course 30 hours. While the early phase of the experiment (

OTHER RELATED OMICS DATASETS IN: PRJNA281173

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Zhe Cheng

LAB HEAD: Christine Vogel

PROVIDER: PXD002039 | Pride | 2016-03-14

REPOSITORIES: Pride

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Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress.

Cheng Zhe Z Teo Guoshou G Krueger Sabrina S Rock Tara M TM Koh Hiromi W L HW Choi Hyungwon H Vogel Christine C

Molecular systems biology 20160120 1

The relative importance of regulation at the mRNA versus protein level is subject to ongoing debate. To address this question in a dynamic system, we mapped proteomic and transcriptomic changes in mammalian cells responding to stress induced by dithiothreitol over 30 h. Specifically, we estimated the kinetic parameters for the synthesis and degradation of RNA and proteins, and deconvoluted the response patterns into common and unique to each regulatory level using a new statistical tool. Overall ...[more]

PMID: 26792871

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