Dataset Information

Phosphotyrosine profiling of curcumin-induced signaling

ABSTRACT: Curcumin, derived from the rhizome of Curcuma longa is a natural anti-cancer agent and has been shown to inhibit proliferation and survival of tumor cells. Although the anti-cancer effects of curcumin are well established, detailed understanding of the signaling pathways altered by curcumin is still unclear. In this study, we carried out SILAC-based quantitative proteomic analysis of CAL 27 cell line, a HNSCC cell line to investigate tyrosine signaling in response to curcumin. Using high resolution Orbitrap Fusion™ Tribrid™ Fourier transform mass spectrometer, we identified 627 phosphotyrosine sites mapping to 357 proteins. We observed alterations in the level of phosphorylation of 307 sites corresponding to 204 proteins upon curcumin treatment.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oral Mucosa Squamous Cell

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD002097 | Pride | 2018-10-24

REPOSITORIES: Pride

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Publications

Phosphotyrosine profiling of curcumin-induced signaling.

Sathe Gajanan G Pinto Sneha M SM Syed Nazia N Nanjappa Vishalakshi V Solanki Hitendra S HS Renuse Santosh S Chavan Sandip S Khan Aafaque Ahmad AA Patil Arun H AH Nirujogi Raja Sekhar RS Nair Bipin B Mathur Premendu Prakash PP Prasad T S Keshava TSK Gowda Harsha H Chatterjee Aditi A

Clinical proteomics 20160615

<h4>Background</h4>Curcumin, derived from the rhizome Curcuma longa, is a natural anti-cancer agent and has been shown to inhibit proliferation and survival of tumor cells. Although the anti-cancer effects of curcumin are well established, detailed understanding of the signaling pathways altered by curcumin is still lacking. In this study, we carried out SILAC-based quantitative proteomic analysis of a HNSCC cell line (CAL 27) to investigate tyrosine signaling in response to curcumin.<h4>Results ...[more]

PMID: 27307780

Dataset Information

Phosphotyrosine profiling of curcumin-induced signaling

Publications

Phosphotyrosine profiling of curcumin-induced signaling.

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