Proteomics

Dataset Information

ER/PR RIME Experiments in MCF7 and T47D cell lines


ABSTRACT: Immuno-precipitation followed by MS was performed using the RIME protocol in this study. Estrogen Receptor (ER) and Progestorone Receptor (PR) were targetted using antibodies. The experiments were performed in a quantitative manner using SILAC labelling. Cells grown in complete serum (estrogenic) conditions were compared against an cells in similar complete media, however supplemented with Progesterone (PG) or R5020 for 4 hours. Experiments were performed in MCF7 and T47D cell lines

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Hisham Mohammed  

LAB HEAD: Dr Jason Carroll

PROVIDER: PXD002104 | Pride | 2015-05-26

REPOSITORIES: Pride

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