ER/PR RIME Experiments in MCF7 and T47D cell lines
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ABSTRACT: Immuno-precipitation followed by MS was performed using the RIME protocol in this study. Estrogen Receptor (ER) and Progestorone Receptor (PR) were targetted using antibodies. The experiments were performed in a quantitative manner using SILAC labelling. Cells grown in complete serum (estrogenic) conditions were compared against an cells in similar complete media, however supplemented with Progesterone (PG) or R5020 for 4 hours. Experiments were performed in MCF7 and T47D cell lines
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell, Cell Culture
DISEASE(S): Breast Cancer
SUBMITTER:
Hisham Mohammed
LAB HEAD: Dr Jason Carroll
PROVIDER: PXD002104 | Pride | 2015-05-26
REPOSITORIES: Pride
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