Proteomics

Dataset Information

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The Proteome of Mouse Vestibular Hair Bundles Over Development


ABSTRACT: Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricular epithelium; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data will be useful for examining the proteins present in mammalian vestibular bundles and how they change over development.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus musculus  

TISSUE(S): Utricle, Inner Ear

DISEASE(S): Not Available

SUBMITTER: Peter Barr-Gillespie  

LAB HEAD: Peter Barr-Gillespie

PROVIDER: PXD002167 | Pride | 2015-08-17

REPOSITORIES: pride

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Publications

Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function.

Krey Jocelyn F JF   Drummond Meghan M   Foster Sarah S   Porsov Edward E   Vijayakumar Sarath S   Choi Dongseok D   Friderici Karen K   Jones Sherri M SM   Nuttall Alfred L AL   Barr-Gillespie Peter G PG  

Scientific reports 20160602


The phospholipid- and Ca(2+)-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, the inner ear's sensory organelle. Using quantitative mass spectrometry, we estimated that ANXA5 accounts for ~15,000 copies per stereocilium, or ~2% of the total protein there. Although seven other annexin genes are expressed in mouse utricles, mass spectrometry showed that none were present at levels near ANXA5 in bundles and none were upregula  ...[more]

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