Proteomics

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PME1 interactome - Relevance Rank Platform (RRP) for Functional Filtering of High Content Protein-Protein Interaction Data

ABSTRACT: For a single step AP-MS identification of PME1 interactome was established a cell line stably expressing either Strep-tag alone or Strep_PME-1 fusion protein. After Strep-tag affinity purification, proteins were separated on SDS-PAGE gel, silver stained and protein bands that showed visually enrichment in StrepPME-1 samples compared to Strep-tag samples were isolated from both lanes for MS analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Anne Rokka  

LAB HEAD: Jukka Westermarck

PROVIDER: PXD002637 | Pride | 2015-12-08

REPOSITORIES: Pride

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Relevance Rank Platform (RRP) for Functional Filtering of High Content Protein-Protein Interaction Data.

Pokharel Yuba Raj YR   Saarela Jani J   Szwajda Agnieszka A   Rupp Christian C   Rokka Anne A   Lal Kumar Karna Shibendra S   Teittinen Kaisa K   Corthals Garry G   Kallioniemi Olli O   Wennerberg Krister K   Aittokallio Tero T   Westermarck Jukka J  

Molecular & cellular proteomics : MCP 20151023 12

High content protein interaction screens have revolutionized our understanding of protein complex assembly. However, one of the major challenges in translation of high content protein interaction data is identification of those interactions that are functionally relevant for a particular biological question. To address this challenge, we developed a relevance ranking platform (RRP), which consist of modular functional and bioinformatic filters to provide relevance rank among the interactome prot  ...[more]

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