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Incorporation of chlorophyll a into Rhodobacter sphaeroides

ABSTRACT: Heterologous expression of a water soluble chlorophyll protein from Brassica oleracea var. gemmifera in the phototrophic purple bacterium Rhodobacter sphaeroides was verified by bottom-up proteomic analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Brassica Oleracea Var. Gemmifera (brussel Sprouts)

TISSUE(S): Photosynthetic Cell

SUBMITTER: Philip Jackson

LAB HEAD: Mark Dickman

PROVIDER: PXD002638 | Pride | 2016-07-12

REPOSITORIES: Pride

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Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll-Protein Complex.

Hitchcock Andrew A Jackson Philip J PJ Chidgey Jack W JW Dickman Mark J MJ Hunter C Neil CN Canniffe Daniel P DP

ACS synthetic biology 20160524 9

Improvements to photosynthetic efficiency could be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption. The development of organisms that can produce both bacteriochlorophylls and chlorophylls is one way to achieve this aim, and accordingly we have engineered the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Bacteriochlorophyll and chlorophyll share ...[more]

PMID: 27171912

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