Dataset Information

Proteomic analysis of prothoracic glands of the silkworm Bombyx mori

ABSTRACT: The project aim was to identify the cell membrane receptors that prothoracic gland cells use to decode and transduce information from the extracellular environment during the final larval stage of the model insect, Bombyx mori.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Molting Gland, Steroid Hormone Secreting Cell

SUBMITTER: Skarlatos Dedos

LAB HEAD: Skarlatos G. Dedos

PROVIDER: PXD002771 | Pride | 2016-01-27

REPOSITORIES: Pride

ACCESS DATA

Publications

Reassessing ecdysteroidogenic cells from the cell membrane receptors' perspective.

Alexandratos Alexandros A Moulos Panagiotis P Nellas Ioannis I Mavridis Konstantinos K Dedos Skarlatos G SG

Scientific reports 20160205

Ecdysteroids secreted by the prothoracic gland (PG) cells of insects control the developmental timing of their immature life stages. These cells have been historically considered as carrying out a single function in insects, namely the biochemical conversion of cholesterol to ecdysteroids and their secretion. A growing body of evidence shows that PG cells receive multiple cues during insect development so we tested the hypothesis that they carry out more than just one function in insects. We cha ...[more]

PMID: 26847502

Similar Datasets

Project description:The high incidence of pre-eclampsia, affecting 5-10% of pregnancies, makes it a major health problem. Early detection of pre-eclampsia, before the appearance of the first clinical symptoms, is therefore an urgent need, since it would allow the early intervention. Identification of plasma/serum biomarkers would pave the way to develop new strategies for the diagnosis of pre-eclampsia. Liquid biopsy emerges as a promising source of protein biomarkers that circumvent some of the inherent challenges of proteome wide analysis of plasma and serum. Besides, purified exosomes have the added interest of being communication vehicles between cells and tissues both in physiological and pathological processes. Methods We compared the protein abundance in purified exosomes from three different cohorts of control and preeclamptic serum samples, obtained around the sixth month and at the end of pregnancy. To this end, a shotgun label-free proteomics analysis was conducted and the relevant differential proteins were then validated by targeted MRM. Results An exosome purification method was developed that yielded highly enriched preparations, as indicated by the detection of a large number of exosomal markers. Likewise, the presence of specific pregnancy protein markers suggested that a very significant percentage of purified exosomes come from tissues related to pregnancy. Shotgun quantitative proteomic analysis allowed us to identify 10, 114 and 98 differentially regulated proteins in the three studied cohorts, with a high degree of concordance among them. Functional analysis suggests that these proteins participate in biological processes closely related to pre-eclampsia, such as angiogenesis, inflammation or cell migration. Differential abundance of 66 proteins were validated by MRM. Finally, we show the impact of the pre-eclampsia associated exosomes in the proteome of a cellular model, compared to normal exosomes. Conclusions We identified and validated differential proteins in liquid biopsy of patients that open new possibilities for the early diagnostic of pre-eclampsia. Additionally, the functional impact of the different proteome composition of purified pre-eclamptic exosomes in target cells provide new information to further understand changes on embryo-maternal interactions and therefore the pathogenesis of this disease.

Dataset Information

Proteomic analysis of prothoracic glands of the silkworm Bombyx mori

Publications

Reassessing ecdysteroidogenic cells from the cell membrane receptors' perspective.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure