Phosphoproteome analysis

ABSTRACT: The phosphatase activirty and dephosphorylation site are determined by using the recombinant protein. After treatment, the level of specific phosphorylation site is decreased. Also, the biological importance is evaluated through a serial of experiments including immunoprecipitation, western blotting, etc,. Based on this workflow, it is possible to determine the tyrosine phosphorylation since the sample complexity is reduced. This finding suggested this phosphatase play a key role in phosphoregulation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Miao-Hsia Lin  

LAB HEAD: miao-hsia lin

PROVIDER: PXD002894 | Pride | 2022-04-13

REPOSITORIES: Pride