Proteomics

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Tomato xylem proteome composition following infection by Fusarium oxysporum


ABSTRACT: The influence of during colonization by Fusarium oxysporum f. sp. Lycopersici secreted effector proteins on the proteome of the xylem sap of tomato plants was investigated using a label-free quantitative proteomics approach. A comparison was made between plants inoculated with either a mock control, a non-effector knockout control, Fusarium oxysporum Fol007 wildtype and four Fol007 single effector protein knockout strains. Specific effects on the relative abundance of certain proteins of the xylem sap occurred for the different knockout strains next to a core set of 24 differentially accumulated proteins which may provide insights into the mechanisms of promoting infection for each of the tested effector proteins.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Solanum Lycopersicum Fusarium Oxysporum F. Sp. Lycopersici

TISSUE(S): Xylem

SUBMITTER: Sjef Boeren  

LAB HEAD: Sjef Boeren

PROVIDER: PXD003010 | Pride | 2015-10-26

REPOSITORIES: Pride

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Publications

The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection.

Gawehns Fleur F   Ma Lisong L   Bruning Oskar O   Houterman Petra M PM   Boeren Sjef S   Cornelissen Ben J C BJ   Rep Martijn M   Takken Frank L W FL  

Frontiers in plant science 20151104


Plant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization of the tomato xylem vessels the fungus Fusarium oxysporum f.sp. lycopersici (Fol) secretes small proteins that are referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3), Six3 (Avr2), Six5, and Six6 are required for full virulence, denoting them as effectors. To investigate their activities in the plant, the xylem sap proteome of plants inoculated with Fol wild-type or  ...[more]

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