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Large-scale in vitro kinase assay with FLNc d23-24 and PKC alpha

ABSTRACT: Large-scale in vitro kinase assay with human and murine FLNc and its site mutants and PKC alpha.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Friedel Drepper

LAB HEAD: Bettina Warscheid

PROVIDER: PXD003185 | Pride | 2017-01-02

REPOSITORIES: Pride

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Myofibrillar Z-discs Are a Protein Phosphorylation Hot Spot with Protein Kinase C (PKCα) Modulating Protein Dynamics.

Reimann Lena L Wiese Heike H Leber Yvonne Y Schwäble Anja N AN Fricke Anna L AL Rohland Anne A Knapp Bettina B Peikert Christian D CD Drepper Friedel F van der Ven Peter F M PF Radziwill Gerald G Fürst Dieter O DO Warscheid Bettina B

Molecular & cellular proteomics : MCP 20161227 3

The Z-disc is a protein-rich structure critically important for the development and integrity of myofibrils, which are the contractile organelles of cross-striated muscle cells. We here used mouse C2C12 myoblast, which were differentiated into myotubes, followed by electrical pulse stimulation (EPS) to generate contracting myotubes comprising mature Z-discs. Using a quantitative proteomics approach, we found significant changes in the relative abundance of 387 proteins in myoblasts <i>versus</i> ...[more]

PMID: 28028127

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