Proteomics

Dataset Information

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Characterisation of pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling

ABSTRACT: Here we use high-resolution mass spectrometry (MS) to characterise the magnitude and complexity of global tyrosine phosphorylation PDAC. We profiled the endogenous tyrosine phosphorylation-based signaling in 36 cell lines from two cell line populations, quantifying around 2,000 tyrosine phosphorylation sites. This approach facilitated consistent segregation of PDAC cell lines into three subtypes with distinct signalling profiles, using both their global tyrosine phosphorylation patterns and a Random Forest derived 8 phosphorylation site signature. We extracted segregation-driving phosphosites, and subsequent pathway and network analysis to characterise the identified subtypes.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pancreatic Cell Line

DISEASE(S): Pancreatic Cancer

SUBMITTER: Emily Humphrey  

LAB HEAD: Professor Roger Daly

PROVIDER: PXD003198 | Pride | 2016-06-09

REPOSITORIES: Pride

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Publications

Resolution of Novel Pancreatic Ductal Adenocarcinoma Subtypes by Global Phosphotyrosine Profiling.

Humphrey Emily S ES   Su Shih-Ping SP   Nagrial Adnan M AM   Hochgräfe Falko F   Pajic Marina M   Lehrbach Gillian M GM   Parton Robert G RG   Yap Alpha S AS   Horvath Lisa G LG   Chang David K DK   Biankin Andrew V AV   Wu Jianmin J   Daly Roger J RJ  

Molecular & cellular proteomics : MCP 20160603 8

Comprehensive characterization of signaling in pancreatic ductal adenocarcinoma (PDAC) promises to enhance our understanding of the molecular aberrations driving this devastating disease, and may identify novel therapeutic targets as well as biomarkers that enable stratification of patients for optimal therapy. Here, we use immunoaffinity-coupled high-resolution mass spectrometry to characterize global tyrosine phosphorylation patterns across two large panels of human PDAC cell lines: the ATCC s  ...[more]

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