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Podophyllum hexandrum Royle MS/MS

ABSTRACT: In order to study the molecular mechanisms of cold-adaption and PPT accumulation to understand why P. hexandrum adapts to chilling temperature, the differentially expressed proteins (DAPs) of P. hexandrum seedling exposed to chilling temperatures (4 and 10°C, 22°C as control) were conducted by using 2-DE in combination with MALDI-TOF-MS, a total of 35 DAP spots were detected and identified. Interestingly, 14 of the 35 DAPs played key roles in PPT biosynthesis, and 21 proteins participated in growth and development. These protein spots were classified into 7 functional categories, including PPT biosynthesis, primary metabolism, transcription and protein synthesis, cell growth/division, defense, transport, and energy. The present data would be useful in better understanding the molecular basis of chilling temperature response in P. hexandrum.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sinopodophyllum Hexandrum

TISSUE(S): Plant Cell, Root

SUBMITTER: Meng-Fei LI

LAB HEAD: LI Wei

PROVIDER: PXD003334 | Pride | 2017-05-15

REPOSITORIES: Pride

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Chilling temperature stimulates growth, gene over-expression and podophyllotoxin biosynthesis in Podophyllum hexandrum Royle.

Yang De Long L Sun Ping P Li Meng Fei MF

Plant physiology and biochemistry : PPB 20160608

Podophyllotoxin (PPT) and its derivatives, isolated from the rhizome of Podophyllum hexandrum Royle (P. hexandrum), are typically used in clinical settings for anti-cancer and anti-virus treatments. Empirical studies have verified that P. hexandrum had stronger tolerance to chilling, due to involving PPT accumulation in rhizome induced by cold stress. However, the cold-adaptive mechanism and its association with PPT accumulation at a molecular level in P. hexandrum are still limited. In this stu ...[more]

PMID: 27314513

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