SCN circadian proteomes of miR-132/212 KO and WT mice by super-SILAC-based quantitative mass spectrometry

ABSTRACT: To identify potential mechanisms underlying the phenotype of miR-132/212 KO mice under short photoperiods, we used quantitative mass spectrometry to analyze the SCN proteomes of miR-132/212 KO and WT mice under a 8:16 LD schedule at 4 time points, spaced 6h apart, across a 24h cycle (n=4 per time point per genotype). Quantification was achieved using a spike-in reference of three murine neural cell lines, including Neuro2A, and the adult mouse hypothalamic cell lines mHypoA-2/28 (CLU188) and mHypoA-SCN mix (CLU497) that had been labeled by SILAC (stable isotope labeling by amino acids in cell culture).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: kerwin chiang  

LAB HEAD: Daniel Figeys

PROVIDER: PXD003635 | Pride | 2017-04-24

REPOSITORIES: Pride