Quantitative proteomics analysis of exosomes purified from serum of patients with colorectal cancer

ABSTRACT: Tumor cells of colorectal cancer (CRC), release exosomes into peripheral blood. These exosomes can mediate communication between cells and affect various tumor-related processes in their target cells. We present a quantitative proteomics analysis on the exosomes purified from serum of patients with CRC and normal volunteers. 918 proteins were identified with an overlap of 725 Gene IDs in Exocarta proteins list. Comparing with the serum-purified exosomes of normal volunteers, we found 36 proteins upregulated and 22 proteins downregulated in the serum-purified exosomes of CRC patients, of which over 50% were involved in protein metabolism and immune response. Pathway and interaction network analysis results revealed that most of the exosome-enriched differentially expressed proteins (DEPs) were associated with complement and coagulation cascades and cancer pathways, suggesting their potential roles in tumor carcinogenesis. Our study demonstrates that serum-purified exosomes of CRC patients can play a pivotal role in invasiveness and pre-metastatic niche establishment of CRC, but not sustain the tumor survival and proliferation. The content selection of exosomes might be associated with the functions of proteins in the development of CRC. This information will be helpful in elucidating the pathophysiological functions of tumor-derived exosomes, and aid in the development of colorectal cancer diagnostics and therapeutics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Colon Cancer

SUBMITTER: Wei Ge  

LAB HEAD: Wei Ge

PROVIDER: PXD003875 | Pride | 2016-12-12

REPOSITORIES: Pride